Precipitating DNA with ethanol

For small ammounts

1. Make up to 270ul with TE.

2. Add 30ul 3M Na acetate.

3. Add 1-2ul Glycogen as a carrier.

4. Add 300ul PCI (Phenol: Chloroform: Isoamyl alcohol).

5. Vortex, centrifuge at 14 000 rpm for 5 min.

6. Remove the upper (aqueous) phase to a fresh tube.

7. Precipitate by adding 2.5 times the volume of ICE COLD 100% ethanol

8. Chill at -20C for at least 30 min. (or - 70C for 10 min.). Procedure can be stoped at this stage and samples stored at - 70C.

9. centrifuge at 14 000 rpm for 15 min. at 4C.

10. Wash pellet in 70% ethanol.

11. Air dry pellet.

12. Resuspend pellet in 10-20ul TE




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