Mini Preps

from a 2 ml culture of bacteria in TB grown overnight

1. Pour 1.5 ml into an eppendorf tube.

2. Centrifuge at 3 000 rpm for 3 min. at room temperature.

3. Discard supernatant.

4. Redisolve pellet in 100ul solution 1.

5. Vortex

6. Add 200 ul solution 2

7. Mix thoroughly by inversion.

8. Put on ice for 5 min.

9. Add 150 ul solution 3

10. Mix thoroughly by inversion.

11. Put on ice for 5 min

12. centrifuge at max speed for 10 min. at 4C.

13. Take off 360 ul supernatant.

14. Transfer into an eppendorf tube with 800 ul 100% EtOH.

15. Store for 5 min. at room temperature.

16. Precipitate by centrifuging at max speed for 15 min. at 4C.

17. Wash pellet with 70% EtOH.

18. Airdry pellet.

19. Redisolve in 20ul TE containing RNAse.

Solution 1: 50 mM glucose, 10 mM EDTA pH8.0, 25 mM Tris pH7.0, autoclave at 100C.

Solution 2: 2% SDS + 0.4 N NaOH , mix these two just before use.

Solution 3: 5M Potassium Acetate.

Writing for MAPK resource
MAP kinase resource community
MAP Kinase Wiki
MAP Kinase Conference 2014

Latest MAP Kinase news

The Biochemistry, Biology and Pathology of MAP Kinases II, September 11-12, 2014, Vilnius, Lithuania

It`s our pleasure to invite you to the international event "The Biochemistry, Biology and Pathology of MAP Kinases II". The conference is organized by Enterprise Lithuania together with Swiss...

Next-Gen Kinase Inhibitors Conference - Cambridge Healthtech Institute, June 17 - 19, 2013

This established and well-recognized kinase conference addresses reoccurring questions about selectivity, safety, resistance and novelty. After many years of research and therapeutic developments of...

Control of the cell cycle progression by the MAPK Hog1

Eukaryotic cells coordinate various intracellular activities in response to environmental stresses, activating an adaptive program to maximize the probability of survival and proliferation. Cells...

Ultrasensitive MAPK/Erk activation in absence of protein synthesis in Xenopus oocytes

The mitogen-activated protein kinase (MAPK) cascade in Xenopus oocytes exhibits an all-or-none, ultrasensitive response, which is believed to result from a positive feed-back loop. Here we describe a...

Batsheva de Rothschild Seminar on Biochemistry, Biology and Pathology of MAP Kinases

This abstract book contains most of the abstracts presented to the Batsheva de Rothschild Seminar on Biochemistry, Biology and Pathology of MAP Kinases. An Aharon Katzir-Katchalski Meeting and an...

Project Sponsors & Friends

Swiss Institute of Bioinformatics Journalism differently - journalism about mass media

Lithuanian Biotechnology Association Enterprise Lithuania

PAGEPress Publications - scholarly open access journals International Society for Biocuration

European Bioinformatics Institute

The Coins