In vitro translation

TNT Rabbit Ret. Lysate 25 ul

TNT Rxn Buffer 2ul

TNT RNA T3/T7 Pol 1ul

Amino Acids (-) Met 1ul

S35 Met 4ul

DEPC dH2O 15ul

Plasmid DNA 1/2ul (1ug)

Rnasin (Promega) 1ul

Mix reagents at 30C for 90 min.

Store at –80C




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