Protocols

Molecular and Cell Biology Protocols

Protocols below are the protocols I've had used and I KNOW THEY WORK!

I did not include protocols for any kit, since most of the kits are sold with protocol included.

DNA and RNA protocols

Restriction Enzyme Digest

Ligation

E. Coli transformation

Precipitating DNA with ethanol

Mini Preps

Protein protocols

Western Blot

In vitro translation

Nuclear Protein Extraction

Coimmunoprecipitation (whole cell lysates)

Coimmunoprecipitation (nuclear extracts)

Cell culture

Cell freezing

Cell lysis



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